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Title: Impedimetric evaluation of hybrid cationic porphyrin/quantum dot multilayer assemblies: a biocompatible interface for calf thymus DNA immobilization
Authors: García, Camilo
Navarro, Fredy
Ruíz-León, Domingo
García-Beltrán, Olimpo
Aguirre, María Jesús
Keywords: Cationic porphyrins
Quantum dots
DNA-porphyrin interactions
Layer by layer assemblies
Calf thymus DNA determination
Issue Date: 10-Jan-2017
Publisher: Journal of Solid State Electrochemistry
Citation: García, C., Navarro, F., Ruíz-León, D. et al. J Solid State Electrochem (2017) 21: 243.
Abstract: In this work, a cationic porphyrin, ascribed as [Ttolyl(P-(C6H5)3)4]4+, where Ttolyl = 5,10,15,20 tetrakistolylporphyrin, was electrostatically assembled with CdTe quantum dots capped with glutathione (GSH) of diameter ~3.28 nm via a layer-by-layer methodology. This multilayer assembly was evaluated as a biocompatible interface with synergic effects for calf thymus double-stranded DNA (CT DNA) immobilization, considering that the hybrid assembly contains a cationic porphyrin with a high binding constant for CT DNA, and quantum dots with polypeptide as a capping agent. The multilayer assembly, ascribed as ITO/{[Ttolyl(P-(C6H5)3)4]4+/CdTe} n (n = 1–5), was characterized by UV-Vis spectroscopy, cyclic voltammetry, and electrochemical impedance spectroscopy (EIS). The last technique was explored in order to check the adsorption of CT DNA onto the multilayer porphyrin/quantum dot assembly. The difference in electron transfer resistance (ΔR ct) obtained after CT DNA incubations showed the best result for a specific multilayer assembly, n = 3. {[Ttolyl(P-(C6H5)3)4]4+/CdTe}3 showed a good correlation of ΔR ct with the logarithmic concentration of CT DNA, in the range 1.0 × 10−10 to 1.0 × 10−6 M with a limit of detection (LOD) of 1.5 × 10−12 M. Conversely, when the {[Ttolyl(P-(C6H5)3)4]4+/CdTe}3 system was incubated with calf thymus single-stranded DNA and salmon testes DNA, no significant difference in ΔR ct was observed. We conclude that the newly described {[Ttolyl(P-(C6H5)3)4]4+/CdTe} n system is a choice method for the impedimetric determination of CT DNA.
ISSN: 1432-8488
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