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The effect of temperature on candidate gene expression in the brain of honey bee Apis mellifera (Hymenoptera: Apidae) workers exposed to neonicotinoid imidacloprid

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dc.contributor.authorManzia, Catalina
dc.contributor.authorVergara-Amado, Jonathan
dc.contributor.authorFranco, Lida M.
dc.contributor.authorSilva, Andrea X.
dc.date.accessioned2020-11-24T22:47:47Z
dc.date.available2020-11-24T22:47:47Z
dc.date.issued2020-08-15
dc.description.abstractNeonicotinoid insecticides are potent agonists of nicotinic acetylcholine receptors and are a major factor in the decline of pollinators worldwide. Several studies show that low doses of this neurotoxin influence honey bee physiology, however, little is known about how insecticides interact with other environmental variables. We studied the effects of two neonicotinoid Imidacloprid doses (IMD, 0, 2.5, and 10 ppb), and three temperatures (20, 28, and 36°C) on gene expression in the brains of worker honey bees (Apis mellifera). Using qRT-PCR we quantified the expression of eight key genes related to the nervous system, stress response, and motor and olfactory capacities. Gene expression tended to increase with the low IMD dose, which was further intensified in individuals maintained in the cold treatment (20°C). At 20°C the octopamine receptor gene (oa1) was underexpressed in bees that were not exposed to IMD, but overexpressed in individuals exposed to 2.5 ppb IMD. Also, heat shock proteins (hsp70 and hsp90) increased their expression at high temperatures (36°C), but not with IMD doses. These results suggest that despite the low insecticide concentrations used in this study (a field-realistic dose), changes in gene expression associated with honey bee physiological responses could be induced. This study contributes to the understanding of how neonicotinoid residual doses may alter honey bee physiology.es_CO
dc.description.sponsorshipUniversidad de Ibaguées_CO
dc.identifier.citationManzi, C., Vergara-Amado, J., Franco, L. M., & Silva, A. X. (2020). The effect of temperature on candidate gene expression in the brain of honey bee Apis mellifera (Hymenoptera: Apidae) workers exposed to neonicotinoid imidacloprid. Journal of Thermal Biology, 102696. doi:10.1016/j.jtherbio.2020.102696es_CO
dc.identifier.issn0306-4565
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S030645652030468X?casa_token=RZbv3zbSU0wAAAAA:pPbZcD8Ug3xe71ZRF1W0bxmaq7ry53VmCADZ0Skn61ref9hdtjyy75hGqgtWlq5QN9lufxtl3GAk
dc.language.isoenes_CO
dc.publisherJournal of Thermal Biologyes_CO
dc.subjectHoney beees_CO
dc.subjectResidual doseses_CO
dc.subjectNeonicotinoides_CO
dc.subjectImidacloprides_CO
dc.subjectTemperaturees_CO
dc.subjectGene expressiones_CO
dc.titleThe effect of temperature on candidate gene expression in the brain of honey bee Apis mellifera (Hymenoptera: Apidae) workers exposed to neonicotinoid imidacloprides_CO
dc.typeArticlees_CO
dspace.entity.typePublication
eperson.emaillida.franco@unibague.edu.coes_CO
eperson.emaillida.franco@unibague.edu.coes_CO
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